High formation of superoxide anion and nitric oxide, and matrix metalloproteinases activity in vascular wall of rectal carcinoma vessels.

AIM

To study the relationship between the level of generation of reactive oxygen species (ROS) and nitric oxide (NO) and activity of matrix metalloproteinases (MMPs) MMP-2 and MMP-9 in the vessels isolated from rectal tumors and Arteria rectalis superior.

METHODS

EPR at the room temperature and 77 degrees K, Spin Traps technology and zymography in polyacrylamide gels were applied.

RESULTS

In the vessels isolated from rectal tumors and Arteria rectalis superior high levels of ROS, NO and formation of complexes of NO with FeS-proteins at the sites of electron-transporting chain of mitochondria have been detected. High activities of MMP-2 and MMP-9 in vascular wall were also observed. The direct positive correlation between the rate of NO generation and formation of complexes of NO with FeS-proteins as well as between ROS and NO formation and MMPs activities have been revealed.

CONCLUSION

Altered oxidative equilibrium in mitochondria of cells in vascular wall promotes formation of cell hypoxia and its autocatalytic potentiation accompanied with activation of MMPs.