B-dependent radical SAM enzymes: Ever expanding structural and mechanistic diversity.

Alhosna Benjdia, Olivier Berteau

Journal: Current opinion in structural biology 2023;83():102725

PMID: 37931378

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Abstract

In the last decade, B-dependent radical SAM enzymes have emerged as central biocatalysts in the biosynthesis of a myriad of natural products. Notably, these enzymes have been shown to catalyze carbon-carbon bond formation on unactivated carbon atoms leading to unusual methylations. Recently, structural studies have revealed unprecedented insights into the complex chemistry catalyzed by these enzymes. In this review, we cover recent advances in our understanding of B-dependent radical SAM enzymes from a mechanistic and structural perspective. We discuss the unanticipated diversity of these enzymes which suggests evolutionary links between various biosynthetic and metabolic pathways from antibiotic to RiPP and methane biosynthesis.

Address: Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, ChemSyBio, 78350, Jouy-en-Josas, France. Electronic address: [email protected].; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, ChemSyBio, 78350, Jouy-en-Josas, France. Electronic address: [email protected].

DOI: 10.1016/j.sbi.2023.102725

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MeSH Terms: Methylation, Carbon, S-Adenosylmethionine, Enzymes