Interferon-beta antibodies have a higher affinity in patients with neutralizing antibodies compared to patients with non-neutralizing antibodies.

C Gneiss, P Tripp, R Ehling, M Khalil, A Lutterotti, R Egg, I Mayringer, B Künz, T Berger, M Reindl, F Deisenhammer

Journal: Journal of neuroimmunology 2006;174(1-2):174-9

PMID: 16556466

Full Text on Elsevier Science View on PubMed

Abstract

In this study, we investigated the affinity, determined by a relative affinity assay, using increasing concentrations of sodium-isothiocyanate to disrupt the antigen antibody binding of neutralizing and non-neutralizing antibodies against interferon-beta (IFNbeta)-1a and -1b in 73 serum samples of MS patients treated with IFNbeta-1a or -1b. Relative affinity values were significantly higher in NAB-positive compared to NAB-negative samples and in samples of IFNbeta-1a-treated patients compared to IFNbeta-1b. A significant positive correlation between relative affinity values and therapy duration indicates affinity maturation as another qualitative factor in IFNbeta neutralization.

Address: Department of Neurology, Innsbruck Medical University, Anichstrasse 35, 6020 Innsbruck, Austria.

DOI: 10.1016/j.jneuroim.2006.01.017

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MeSH Terms: Analysis of Variance, Antibodies, Binding, Competitive, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Humans, Immunotherapy, Interferon-beta, Multiple Sclerosis, Neutralization Tests, Time Factors, Treatment Outcome