A disposable immunosensor for the detection of salivary MMP-8 as biomarker of periodontitis.

Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T K Thanh, Riccarda Antiochia

Journal: Bioelectrochemistry (Amsterdam, Netherlands) 2024;156():108590

PMID: 37976772

Full Text on Elsevier Science View on PubMed

Abstract

This work describes the development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care. The electrochemical platform was based on a graphene (GPH) screen-printed electrode (SPE) functionalized by gold-nanospheres (AuNSs) and antibodies against MMP-8 protein (anti-MMP-8). The functionalization with anti-MMP-8 was realized by using 11-mercaptoundecanoic acid (11-MUA), thanks to its ability to give strong sulfur bonds with its -SH end, and to cross-link the -NH groups of the antibody molecule with the other -COOH end, using the traditional EDC-NHS method. The voltammetric sensor showed good performances with a linear range of 2.5-300 ng mL, a LOD value of 1.0 ± 0.1 ng mL and a sensitivity of 0.05 µA mL cm ng. Moreover, the proposed immunosensor was tested in real saliva samples, showing comparable results to those obtained with the conventional ELISA method. The biosensor was single-use and cost-effective and required a small quantity of test medium and a short preparation time, representing a very attractive biosensor for MMP-8 detection in human saliva.

Address: Department of Experimental Medicine, Sapienza University of Rome, Viale Regina Elena 324, 00161 Rome, Italy.; Faculty of Chemistry and Biology, Department of Materials Chemistry, University of Santiago of Chile, Av. Libertador Bernardo ÓHiggins 3363, 8320000 Estacion Central, Santiago, Chile.; Biophysics Group, Department of Physics & Astronomy, University College London, Gower Street, London WC1E 6BT, UK; UCL Healthcare Biomagnetic and Nanomaterials Laboratories, Royal Institution of Great Britain, 21 Albermarle Street, London W1S 4BS, UK.; Department of Chemistry and Drug Technologies, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy. Electronic address: [email protected].

DOI: 10.1016/j.bioelechem.2023.108590

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MeSH Terms: Humans, Matrix Metalloproteinase 8, Immunoassay, Biosensing Techniques, Electrochemical Techniques, Limit of Detection, Biomarkers, Periodontitis, Electrodes, Gold