Neomycin improves cationic lipid-mediated transfection of DNA in human cells.

Sara Napoli, Giuseppina M Carbone, Carlo V Catapano, Nick Shaw, Dev P Arya

Journal: Bioorganic & medicinal chemistry letters 2005;15(14):3467-9

PMID: 15950473

Full Text on Elsevier Science View on PubMed

Abstract

Delivery of oligonucleotides has been a major impediment in the development of nucleic acid based drugs. In this report, we show that neomycin, an aminoglycoside antibiotic, when combined with a cationic lipid preparation such as DOTAP, enhances transfection efficiency of both reporter plasmids and oligonucleotides and results in a significant increase in transgene expression. The results described here open a new lead in ongoing efforts for oligonucleotide delivery.

Address: Laboratory of Experimental Oncology, Oncology Institute of Southern Switzerland (IOSI) Via Vela 6, 6500 Bellinzona, Switzerland.

DOI: 10.1016/j.bmcl.2005.04.038

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MeSH Terms: Carbohydrate Sequence, Cations, Cell Line, Tumor, DNA, Drug Delivery Systems, Fatty Acids, Monounsaturated, Humans, Lipids, Male, Molecular Sequence Data, Molecular Structure, Neomycin, Prostatic Neoplasms, Quaternary Ammonium Compounds, Transfection