Lipid rafts clustering and signalling by listeriolysin O.

S Weiss, N O Gekara

Journal: Biochemical Society transactions 2005;32(Pt 5):712-4

PMID: 15493995

Full Text on Silverchair Information Systems View on PubMed

Abstract

Listeriolysin O, the major virulent determinant of Listeria monocytogenes, is known for forming pores on cholesterol-rich membranes. In the present study, we reveal its other facet, rafts clustering. By immunofluorescence microscopy, we show that the glycosylphosphatidylinositol-anchored proteins CD14 and CD24, which normally exhibit uniform distribution on J774 cells, undergo clustering upon treatment with LLO. The non-raft marker transferrin receptor is unaffected by such treatment. Rafts clustering might explain the induction of tyrosine phosphorylation observed on LLO-treated cells.

Address: Molecular Immunology, GBF, German Research Centre for Biotechnology, Mascheroder Weg 1, D-38124 Braunschweig, Germany. [email protected]

DOI: 10.1042/BST0320712

Link outs

Free resources

Molecular Biology Databases:

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-CD14-1

Subscription / membership required

Full Text Sources:

https://portlandpress.com/biochemsoctrans/article-...

MeSH Terms: Antigens, CD, Bacterial Toxins, CD24 Antigen, Cell Line, Cell Membrane, Cholesterol, Heat-Shock Proteins, Hemolysin Proteins, Humans, Lipopolysaccharide Receptors, Listeria monocytogenes, Membrane Glycoproteins, Membrane Microdomains, Microscopy, Fluorescence, Models, Biological, Phosphorylation, Signal Transduction, Tyrosine