An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.

Luana Ugahary, Wendy van de Sande, Jan C van Meurs, Alex van Belkum

Journal: Journal of clinical microbiology 2005;42(11):5403-5

PMID: 15528756

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Abstract

General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

Address: The Rotterdam Eye Hospital, Rotterdam, The Netherlands.

DOI: 10.1128/JCM.42.11.5403-5405.2004

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MeSH Terms: Anti-Infective Agents, Local, DNA, Bacterial, Endophthalmitis, Equipment Contamination, Eye Infections, Bacterial, Humans, Povidone-Iodine, Pseudomonas, Vitrectomy