Technical issues in construction of nucleic acid vaccines.

Peter F Ertl, Lindy L Thomsen

Journal: Methods (San Diego, Calif.) 2004;31(3):199-206

PMID: 14511952

Full Text on Elsevier Science View on PubMed

Abstract

Delivery of antigen-encoding genes by nucleic acid vaccine vectors offers tremendous advantages in power and flexibility over conventional antigen delivery systems. Of the many forms of nucleic acid vaccine that can be constructed, circular DNA plasmids are the simplest. In this article, we consider the components that make up a generic DNA plasmid vaccine. We discuss some of the issues encountered when optimizing these vectors to exploit their potential for in vivo expression and presentation of antigens and thereby maximizing the immune response.

Address: Department of Immunotherapeutics, GlaxoSmithKline, Gunnels Wood Road, Stevenage, SG1 2NY Hertfordshire, UK.

DOI: 10.1016/s1046-2023(03)00134-8

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MeSH Terms: Amino Acid Sequence, Antigens, Base Sequence, Gene Expression, Genetic Vectors, Humans, Molecular Sequence Data, Plasmids, Vaccines, DNA