A confound-free method to manipulate pupil size in psychological experiments.

Researchers are increasingly showing interest in the ways in which various cognitive processes are influenced by the size of the pupil. However, this realm of research is complicated by the pupil's notorious susceptibility to confounders and difficulties in disentangling cause and effect. Recent studies have sought a solution in the exploitation of intrinsically photosensitive retinal ganglion cells (ipRGCs), which trigger pupil constrictions upon detecting blue light. The crux of the method is that stimuli are presented against blue versus red backgrounds of equal perceived luminance, to effectuate systematically smaller pupils in the former compared with the latter condition. Here we provide a further validation of this method, by testing a scenario of potential concern. Via retino-hypothalamic pathways, ipRGC activation modulates alertness and arousal. Blue and red backgrounds may therefore differentially impact behavior in addition to the pupil, potentially confounding inferences about the pupil's impact on cognition. This was investigated with an auditory task in which participants responded as quickly as possible to sequences of randomly timed beeps while looking at blue versus red displays. Each participant was tested with an easy and a difficult version of the auditory task. Results suggest that the ipRGC method is "good to go": in the presence of effects of task difficulty on both pupil size and task performance, the display color exclusively influenced pupil size without affecting task performance.

© 2026. The Author(s).