WHICH ORAL HYGIENE PROTOCOL SHOULD BE FOLLOWED BY PATIENTS WITH FIXED ORTHODONTIC APPLIANCES? A RANDOMIZED CLINICAL TRIAL.

Mohammed A Elbanna, Shahira A Elbanna, Fatma Abdelsayed, Mohamed Abd-El-Ghafour

Journal: The journal of evidence-based dental practice 2025;25(4):102192

PMID: 41290275

Abstract

OBJECTIVE

The aim of this study was to compare the effect of three different oral hygiene protocols on the amount of Streptococcus mutans (S. mutans) colonization in adult patients with fixed orthodontic appliance.

METHODS

A total of 27 patients who fulfilled the eligibility criteria were randomly allocated in the study. After bonding of the fixed appliance, patients were randomly allocated into 3 groups, Group I (conventional manual toothbrushing with fluoridated toothpaste), Group II (Conventional manual toothbrushing combined with interdental brushes) and Group III (Conventional manual toothbrushing combined with interdental brushes and chlorhexidine (CHX) mouthwash). Saliva samples were collected from each patient at two timelines; Before bonding (T0) and 1 month after bonding (T1), then sent for microbial analysis to calculate S. mutans viable count (CFU/mL).

RESULTS

T1 vs T0: Group I showed significant increase in S. mutans viable counts (P = .0117), Group II showed no significant changes in S. mutans levels (P = .425), while Group III showed significant decrease in S. mutans viable counts (P = 0.0078). Upon comparing the three oral hygiene protocols, both group II and group III exhibited prominent impact on oral hygiene whether by maintaining or reducing S. mutans levels, respectively.

CONCLUSION

Conventional manual toothbrush combined with interdental brush protocol with or without CHX were efficient protocols for improving oral hygiene in orthodontic patients. Using conventional manual toothbrushes alone was the least effective oral hygiene protocol. Registration NCT05016713. The protocol was published before trial commencement.

Copyright © 2025 Elsevier Inc. All rights reserved.

Address: Department of Orthodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt.; Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Department of Microbiology and Immunology, School of Pharmacy, Newgiza University, Giza, Egypt.; Department of Orthodontics, Faculty of Dentistry, Cairo University, Cairo, Egypt. Electronic address: [email protected].
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