Cu-curcumin nanozyme for detection of Cr(VI) through an off-on strategy based on peroxidase mimicking activity.

Lingbo Qu, Zhaohui Li, Jingjing Huang, Lin Zhang, Ziwei Chai, Aifang Zhou, Jia Ge

Journal: The Analyst 2025;150(2):371-377

PMID: 39670858

Full Text on Royal Society of Chemistry View on PubMed

Abstract

Hexavalent chromium ions (Cr(VI)) possess inherent toxicity and propensity for bioaccumulation in the environment. Herein, a Cu-curcumin nanozyme was synthesized a one-pot solvothermal method. Based on the high peroxidase-like activity of the Cu-curcumin nanozyme, a novel and economical colorimetric platform has been developed for Cr(VI) assay. In the presence of hydrogen peroxide (HO), the Cu-curcumin nanozyme can catalyze the transformation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) into the blue product oxTMB. 8-Hydroxyquinoline (8-HQ) can inhibit the catalysis of the Cu-curcumin/TMB/HO system, causing the blue color to fade. With the addition of Cr(VI), the peroxidase-like activity of the Cu-curcumin nanozyme is increased significantly, leading to the restoration of the Cu-curcumin/TMB/HO system color. After optimization of the experimental parameters, a novel colorimetric sensor for Cr(VI) assay has been successfully developed with a low limit of detection (LOD) of 31.5 nM, affirming its potential for accurate Cr(VI) quantification in environmental specimens. In addition, paper strips integrated with a smartphone platform enhance the versatility and portability of the Cr(VI) assay, achieving an LOD of 0.1 μM.

Address: College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Food Laboratory of Zhongyuan, Zhengzhou University, Zhengzhou 450001, China. [email protected].; College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Food Laboratory of Zhongyuan, Zhengzhou University, Zhengzhou 450001, China. [email protected].; Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation, Henan Insitute of Food and Salt Industry Inspection Technology, Zhengzhou, 450003, China.; College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Food Laboratory of Zhongyuan, Zhengzhou University, Zhengzhou 450001, China. [email protected].; The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.

DOI: 10.1039/d4an01358a

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https://doi.org/10.1039/d4an01358a

MeSH Terms: Copper, Chromium, Curcumin, Hydrogen Peroxide, Colorimetry, Benzidines, Limit of Detection, Peroxidase, Catalysis, Biomimetic Materials, Nanostructures, Oxyquinoline