New strategies to advance plant transformation.

Kankshita Swaminathan, Can Baysal, Mohammad B Belaffif, Morgan C Brown, Brenda Marcial

Journal: Current opinion in biotechnology 2025;91():103241

PMID: 39732097

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Abstract

Plants are an important source of food, energy, and bioproducts. Advances in genetics, genomics-assisted breeding, and biotechnology have facilitated the combining of desirable traits into elite cultivars. To ensure sustainable crop production in the face of climate challenges and population growth, it is essential to develop and implement techniques that increase crop yield and resilience in environments facing water scarcity, nutrient deficiencies, and other abiotic and biotic stressors. Plant transformation and genome editing are critical tools in the development of new cultivars. Here, we discuss recent advances in plant transformation technologies aimed at enhancing efficiency, throughput, and the number of transformable genotypes. These advancements include the use of morphogenic regulators, virus-mediated genetic modifications, and in planta transformation with Rhizobium rhizogenes.

Address: HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA; Center for Advanced Bioenergy and Bioproducts Innovation, 1206 W. Gregory Drive (IGB), Urbana, IL 61801, USA; Agria Analytica, CIBIS NINE 11th Floor, Jl.TB Simatupang No.2, Jakarta Selatan, DKI Jakarta 12560, Indonesia.; HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA.; HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA; Center for Advanced Bioenergy and Bioproducts Innovation, 1206 W. Gregory Drive (IGB), Urbana, IL 61801, USA; University of Alabama at Huntsville, 301 Sparkman Drive, Huntsville, AL 35899, USA.; Center for Advanced Bioenergy and Bioproducts Innovation, 1206 W. Gregory Drive (IGB), Urbana, IL 61801, USA; Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN 55108, USA.; HudsonAlpha Institute for Biotechnology, 601 Genome Way, Huntsville, AL 35806, USA; Center for Advanced Bioenergy and Bioproducts Innovation, 1206 W. Gregory Drive (IGB), Urbana, IL 61801, USA. Electronic address: [email protected].

DOI: 10.1016/j.copbio.2024.103241

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MeSH Terms: Plants, Genetically Modified, Gene Editing, Transformation, Genetic, Crops, Agricultural, Plant Breeding