Chromosome-scale haploid genome assembly of Durio zibethinus KanYao.

Fengzhi Liu, Xiaodi Wang, Meng Shi, Haibo Wang, Xiaohao Ji, Yiwang Zhong, Daojun Zheng, Shenghua Xie, Xuejie Feng

Journal: Scientific data 2025;12(1):384

PMID: 40044694

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Abstract

Durian (Durio zibethinus) is a tropical fruit valued for its nutritional and commercial significance. In this study, we generated two high-quality, haplotype-resolved, chromosome-level genomes of the durian cultivar 'Kan Yao', each with 28 chromosomes. The total genome lengths were 737.2 Mb and 763.8 Mb, with contig N50 values of 22.9 Mb and 21.5 Mb, and scaffold N50 values of 25.9 Mb and 26.7 Mb, respectively. Nineteen chromosomes were assembled without gaps, while the remaining nine contained 1 to 10 gaps. Genome annotation identified 53,125 and 53,101 functional genes, as well as 5,254 and 5,496 non-coding RNAs. The high-quality assembled genomes will aid in the molecular breeding of durian.

Address: National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, 572024, Hainan, China.; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Xingcheng, 125100, Liaoning, China.; Sanya Institute, Hainan Academy of Agricultural Sciences; Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences; Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Key Laboratory of Tropical Fruit Tree Biology of Hainan Province; Ministry of Agriculture and Rural Affairs/Haikou Scientific Observation and Experimental Station for Tropical Fruit Trees, Hainan Field Scientific Observation and Research Station for Tropical Fruit Trees, Haikou, 571100, Hainan, China.; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Xingcheng, 125100, Liaoning, China.; Sanya Institute, Hainan Academy of Agricultural Sciences; Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences; Key Laboratory of Genetic Resources Evaluation and Utilization of Tropical Fruits and Vegetables (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs; Key Laboratory of Tropical Fruit Tree Biology of Hainan Province; Ministry of Agriculture and Rural Affairs/Haikou Scientific Observation and Experimental Station for Tropical Fruit Trees, Hainan Field Scientific Observation and Research Station for Tropical Fruit Trees, Haikou, 571100, Hainan, China. [email protected].; National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, 572024, Hainan, China. [email protected].; Research Institute of Pomology, Chinese Academy of Agricultural Sciences/Key Laboratory of Horticultural Crops Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Xingcheng, 125100, Liaoning, China. [email protected].

DOI: 10.1038/s41597-025-04656-y

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https://assays.cancer.gov/CPTAC-1474

MeSH Terms: Haploidy, Genome, Plant, Chromosomes, Plant, Molecular Sequence Annotation, Haplotypes