Boosting stability: a hierarchical approach for self-assembling peptide structures.

Marlena Gąsior-Głogowska, Dominika Bystranowska, Natalia Szulc, Monika Szefczyk, Roksana Kruszakin, Denys Balandin

Journal: Journal of materials chemistry. B 2024;12(41):10682-10691

PMID: 39314115

Full Text on Royal Society of Chemistry View on PubMed

Abstract

The primary objective of this study was to implement a hierarchical approach to enhance the conformational stability of a selected group of peptides by incorporating -(1,2)-2-aminocyclopentanecarboxylic acid (-ACPC). The influence of residue mutation on the peptide structures was investigated using circular dichroism, analytical ultracentrifugation, and vibrational spectroscopy. The resulting nanostructures were examined transmission electron microscopy. The incorporation of -ACPC led to increased conformational stability and self-assembling propensity in peptides containing constrained β-amino acid residues.

Address: Department of Bioorganic Chemistry, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland. [email protected].; Department of Medicinal Chemistry, Otto Loewi Research Center, Medical University of Graz, Neue Stiftingtalstrasse 6, 8010, Graz, Austria.; Department of Physics and Biophysics, Wrocław University of Environmental and Life Sciences, Norwida 25, Wrocław 50-375, Poland.; Department of Biochemistry, Molecular Biology and Biotechnology, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland.; Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland.; Laboratory of Instrumental Analysis and Preparation, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Weigla 12, Wrocław 53-114, Poland.; Department of Bioorganic Chemistry, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland. [email protected].

DOI: 10.1039/d4tb01545b

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https://doi.org/10.1039/d4tb01545b

MeSH Terms: Peptides, Nanostructures, Protein Stability