Oregano polyphenols reduce human insulin amyloid aggregation.

Human insulin may undergo fibrillization under specific conditions, impairing its function and promoting its accumulation in amyloid deposits. Oregano (Origanum vulgare L.) leaves are rich in biologically active compounds such as polyphenols. Thus, we investigated their ability to inhibit insulin amyloid aggregation. The oregano aqueous extract phytochemical analysis (LC-MS/MS-DAD) revealed the presence of four major compounds: lithospermic acid (LA), rosmarinic acid (RA), oreganol A (OA), and luteolin-7-O-diglucuronide (L7dG), respectively. Compounds and their mixtures were subsequently screened for anti-amyloid activity and evaluated against oregano lyophilizate (LYO) utilizing ThT assay, AFM and ATR-FTIR analyses. LYO inhibited insulin fibrillization more effectively than its main constituent RA, prolonging the lag phase approximately two-fold. L7dG has been the most effective of the tested individual compounds, prolonging the lag phase by roughly 20 %, followed by LA, whereas OA was ineffective. Subsequently, we measured the anti-amyloid activity of two kinds of equimolar mixtures: either containing individually active compounds or analogous mixtures to which inactive OA was added. Surprisingly, LA:OA mixture proved to be the most effective. However, adding L7dG to the OA mixtures led to activity loss. The interactions of oregano polyphenols with the amyloidogenic regions of insulin were elucidated using molecular docking, explaining observed changes in their anti-amyloid activity. We conclude that when investigating the anti-amyloid activity of samples of natural origin and determining the activity of the extracts and their individual main components, it is necessary to consider their mutual interactions, which can significantly affect the final effect of the analyzed mixture.

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