Designing mouthwash formulations with innovative molecular components to control initial dental erosion in vivo.

Talita Mendes Oliveira Ventura, Larissa Tercilia Grizzo Thomassian, Vinícius Taioqui Pelá, Even Akemi Taira, Eduardo Pereira de Souza, Thiago Saads Carvalho, Flávio Henrique-Silva, Marília Afonso Rabelo Buzalaf, Adrian Lussi, Mariana Miranda de Brito, Gustavo Cassalate da Silva, Carolina Ruis Ferrari, Thais Fernanda Carlos, Andrea Soares da Costa Fuentes, Karina Fan, Reinaldo Marchetto

Journal: Clinical oral investigations 2024;28(12):640

PMID: 39532751

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Abstract

OBJECTIVE

This study aimed to examine and compare the efficacy of mouthwashes containing different proteins and peptide on the prevention of enamel erosion in vivo, as well as to evaluate the participants' satisfaction with the formulations.

METHODS

Twelve participants were selected and underwent five cross-over mouthwash phases: Water (control); 0.1 mg/mL CaneCPI-5; 0.5 mg/mL MaquiCPI-3; 0.1 mg/mL CsinCPI-2; and 0.037 mg/mL Stn15pSpS. After prophylaxis, the participants rinsed (1 min), followed by the acquired enamel pellicle (AEP) formation (2 h). An erosive challenge was made (biopsy, citric acid 1%, 15s) on the buccal surface of the central maxillary incisors. The Relative Surface Reflection Intensity (%SRI) was assessed and analyzed by ANOVA/Tukey's tests. The calcium release in acid was measured by the Arsenazo method and verified by Kruskal-Wallis/Dunn's tests. The Spearman's correlation was used between analyses. A questionnaire evaluated the satisfaction of participants.

RESULTS

For both analyses, the results showed that mouthwashes containing the proteins or peptide were significantly more effective in preventing enamel erosion compared to deionized water, with no significant differences among the active ingredients (p < 0.05). Also, there was a significant negative correlation between %SRI and calcium released (r=-0.5754). The questionnaire revealed that the volunteers were satisfied with the taste of the products. In addition, the experimental procedures were well tolerable, and no side effects were reported.

CONCLUSION

All mouthwashes containing proteins or peptide were acceptable and effective in protecting enamel against initial dental erosion in vivo.

CLINICAL SIGNIFICANCE

This study highlights the potential of these pioneer organic components for the development of mouthwashes designed for people with risk of erosive tooth wear.

Address: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75., Bauru, São Paulo, 17012-901, Brazil.; Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, São Paulo, Brazil.; Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland.; Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.; Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland.; University Hospital for Conservative Dentistry and Periodontology, Medical University of Innsbruck, Innsbruck, Austria.; Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75., Bauru, São Paulo, 17012-901, Brazil. [email protected].

DOI: 10.1007/s00784-024-06040-3

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https://dx.doi.org/10.1007/s00784-024-06040-3

MeSH Terms: Mouthwashes, Humans, Tooth Erosion, Cross-Over Studies, Male, Female, Adult, Surveys and Questionnaires, Citric Acid, Peptides, Patient Satisfaction, Incisor, Dental Pellicle