Intranasal immunization of humans with Streptococcus mutans antigens.

F Li, S M Michalek, A P Dasanayake, Y Li, K Kirk, N K Childers

Journal: Oral microbiology and immunology 2003;18(5):271-7

PMID: 12930517

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Abstract

To evaluate the effectiveness of a low dose of soluble or liposomal (L) glucosyltransferase-enriched preparation (E-GTF) in inducing mucosal immune responses after intranasal immunization, 12 adults were immunized on days 0 and 7 by the IN route with 62.5 microg of soluble E-GTF or L-E-GTF. An increase in the mean salivary IgA anti-E-GTF response (P < 0.03) was seen in the L-E-GTF but not the soluble E-GTF group. A significant increase (P < 0.05) in the mean specific IgA antibody activity was also seen in nasal wash from both groups. Although the nasal wash responses were higher in the L-E-GTF than in the soluble E-GTF group, they were not significantly different. The soluble E-GTF immunized group showed a higher serum IgG response than the L-E-GTF immunized group on day 90 (P < 0.05). These results indicate that as little as 62.5 microg of E-GTF, when given by the intranasal route, induced an IgA response in secretions.

Address: Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, AL 35294-0007, USA.

DOI: 10.1034/j.1399-302x.2003.00067.x

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MeSH Terms: Administration, Intranasal, Adult, Antibodies, Bacterial, Antigens, Bacterial, Glucosyltransferases, Humans, Immunity, Mucosal, Immunization, Immunoglobulin A, Immunoglobulin A, Secretory, Immunoglobulin G, Liposomes, Middle Aged, Nasal Lavage Fluid, Solubility, Streptococcus mutans