Adsorption characteristics of sulfate reducing bacteria Clostridium sp. on lignite surface.

Bo Fu, Huan He, Yong Zhang, Zi Yang Zhou, Di Zhan, Wen Cheng Xia, Feng Juan Lan, Xiu Xiang Tao, Zai Xing Huang

Journal: Environmental science and pollution research international 2025;32(17):11052-11062

PMID: 40195223

Abstract

Biogenic coal bed methane has attracted great attention in recent years. During the process of biogas production, the interaction between microorganisms and coal is a crucial step. Sulfate-reducing bacteria (SRB) play an important role in biogas production. However, the interaction between SRB and coal has always remained an open problem. In the present work, the SRB strain Clostridium sp. and lignite were used to investigate the adsorption process with the extended DLVO (XDLVO) theory, calorimetry, and scanning electron microscopy (SEM). The results showed that the adsorption rate has a positive correlation with pH when it went from 3 to 8. XDLVO theoretical analysis was in good agreement with the adsorption experimental result. Acid-base potential energy is a more critical factor driving the adsorption comparing with electrostatic potential energy and Lifshitz-van Der Waals potential energy. The adsorption process of Clostridium sp. cells on lignite surface can be divided into three main stages: the direct adsorption, or reversible adsorption; desorption process; and irreversible adsorption. From the SEM results, the intercellular cohesion is also a very important adsorption form. The morphology and roughness of coal surface may also have a key effect on adsorption. Overall, our results provide some insights into the surface energy changes of Clostridium sp. adsorbed on coal and their interactions from the perspective of adsorption kinetics.

Address: Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, 221116, China. [email protected].; Key Laboratory of Coal Processing and Efficient Utilization of Ministry of Education, School of Chemical Engineering and Technology, China University of Mining and Technology, Xuzhou, 221116, China.; School of Environmental and Civil Engineering, Jiang Nan University, Wuxi, 214122, China.; Key Laboratory of Coal Bed Methane Resource & Reserving Process of the Ministry of Education, School of Resources and Geosciences, China University of Mining and Technology, Xuzhou, 221008, China.; Center for Biogenic Natural Gas Research, Department of Civil and Architectural Engineering, University of Wyoming, Laramie, WY, 82071, USA.; Department of Chemical and Petroleum Engineering, University of Wyoming, Laramie, WY, 82071, USA.

DOI: 10.1007/s11356-025-36319-1

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https://dx.doi.org/10.1007/s11356-025-36319-1

MeSH Terms: Clostridium, Coal, Adsorption, Sulfates