Advances in biosynthesis of 7-Dehydrocholesterol through de novo cell factory strategies.

Muhammad Israr, Yuchen Han, Wuyuan Zhang, Huanhuan Li, Yawen Huang

Journal: Bioresource technology 2025;418():131888

PMID: 39603472

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Abstract

7-Dehydrocholesterol (7-DHC) is an important sterol for maintaining human health and is present in the skin. After sun exposure, 7-DHC in the skin is converted to vitamin D3 to strengthen the immune system. In recent years, synthetic biology has gained importance due to the effective and efficient production of various important compounds using microorganisms. Despite the understanding of the mechanisms and pathways of 7-DHC biosynthesis, achieving higher production yields remains a significant challenge. This review aims to provide a comprehensive overview of the current state of 7-DHC biosynthesis. Various synthetic strategies including optimization of rate-limiting enzymes, metabolic fluxes, redox balance, and subcellular localization are discussed. Moreover, the role of omics technology in designing important proteins and gene editing techniques for strain modification to efficiently synthesize 7-DHC will also be discussed.

Address: University of Chinese Academy of Sciences, Beijing 100049, PR China; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin 300308, PR China.; State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Street, Wuhan 430070, PR China.; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin 300308, PR China; National Innovation Center for Synthetic Biotechnology, 32 West 7th Avenue, Tianjin 300308, PR China. Electronic address: [email protected].; University of Chinese Academy of Sciences, Beijing 100049, PR China; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin 300308, PR China; National Innovation Center for Synthetic Biotechnology, 32 West 7th Avenue, Tianjin 300308, PR China. Electronic address: [email protected].

DOI: 10.1016/j.biortech.2024.131888

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MeSH Terms: Dehydrocholesterols, Humans, Synthetic Biology, Metabolic Engineering