Recent Advances in Genetic Engineering Strategies of .

Huan Fang, Dawei Zhang, Zhaoxia Jin, Xia Yang, Xinyi Ren, Gang Fu, Huiying Wang, Xuan Liu, Tingting Li, Huina Dong, Miaomiao Xia, Guangqing Du

Journal: ACS synthetic biology 2024;13(11):3497-3506

PMID: 39481116

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Abstract

is a free-living soil Gram-negative bacterium that participates in nitrogen-fixation symbiosis with several legumes. has the potential to be utilized for the production of high-value nutritional compounds, such as vitamin B. Advances in gene editing tools play a vital role in the development of strains with enhanced characteristics for biotechnological applications. Several novel genetic engineering strategies have emerged in recent years to investigate genetic modifications in . This review provides a comprehensive overview of the mechanism and application of the extensively used Tn5-mediated genetic engineering strategies. Strategies based on homologous recombination and site-specific recombination were also discussed. Subsequently, the development and application of the genetic engineering strategies utilizing various CRISPR/Cas systems in are summarized. This review may stimulate research interest among scientists, foster studies in the application areas of , and serve as a reference for the utilization of genome editing tools for other Rhizobium species.

Address: School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; University of Chinese Academy of Sciences, Beijing, 100049, China.; Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.; School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.

DOI: 10.1021/acssynbio.4c00348

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MeSH Terms: Sinorhizobium meliloti, Genetic Engineering, Gene Editing, CRISPR-Cas Systems, DNA Transposable Elements, Homologous Recombination, Symbiosis