Structured protein probes modified with selenium nanoparticle for 1-minute measurement of SARS-CoV-2 antigen.

Sima Yaron, Chunxia Chen, Yang Luo, Rui Chen, Keying Guo, Shujie Duan, Jianing Ji, Mengli Wu, Zhan Yang, Mengyang Cai, Menghan Xue, Lanju Wang, Sergio Benardini, Zhizeng Wang

Journal: Biosensors & bioelectronics 2024;268():116878

PMID: 39499971

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Abstract

Conventional point-of-care lateral flow immunoassays are characterized by an antibody-tagged probe irregular coupling that can limit sensitivity and require a long assay's time. We synthesized polyethylene glycol-modified selenium nanoparticles (PEG-SeNPs) by template method and developed a strategy to set antibody probes targeted and orderly by using PEG-SeNPs. Synthesized PEG-SeNPs with high stability could immobilize antibodies in the "stand-up" orientation, resulting in a faster detection time of less than 1 min by direct observer visualization without instruments or equipment. Results showed that SARS-CoV-2 antibody could be systematically structured on the chip, resulting in a detection limit of 10 pg/mL, significantly higher than conventional chips. The new device has been validated on 192 clinical samples and we found 100% negative coincidence, 93.94% positive coincidence, and 95.83% overall coincidence with reverse transcriptional PCR test. The orderly arrayed probe's stability allowed to detect throat swabs, saliva, serum, fingertip blood samples, and mutant strains without cross-reactivity with common respiratory viruses or pathogenic strains, demonstrating promising potential for a universal colorimetric platform for ultrafast field-deployable diagnostics.

Address: Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, School of Medicine, Henan University, Kaifeng, 475004, People's Republic of China.; Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, School of Medicine, Henan University, Kaifeng, 475004, People's Republic of China; Medical Laboratory Department, Zhumadian Center Hospital, Zhumadian, 463000, People's Republic of China.; Department of Blood Transfusion, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, 450008, People's Republic of China.; Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People's Republic of China.; Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology (IIT), Haifa, 3200003, Israel.; Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, 3052, Australia; Guangdong Provincial Key Laboratory of Materials and Technologies for Energy Conversion (MATEC), Guangdong Technion-Israel Institute of Technology, Shantou, 515063, People's Republic of China. Electronic address: [email protected].; Department of Laboratory Medicine, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing, 401147, People's Republic of China; Department of Experimental Medicine, University of Tor Vergata, Rome, Italy. Electronic address: [email protected].; Joint National Laboratory for Antibody Drug Engineering, Clinical Laboratory of the First Affiliated Hospital, School of Medicine, Henan University, Kaifeng, 475004, People's Republic of China; Department of Laboratory Medicine, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing, 401147, People's Republic of China. Electronic address: [email protected].; Department of Laboratory Medicine, Chongqing General Hospital, School of Medicine, Chongqing University, Chongqing, 401147, People's Republic of China; College of Life Science and Laboratory Medicine, Kunming Medical University, Kunming, Yunnan, 650050, People's Republic of China. Electronic address: [email protected].

DOI: 10.1016/j.bios.2024.116878

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https://assays.cancer.gov/CPTAC-438

MeSH Terms: SARS-CoV-2, Humans, COVID-19, Selenium, Biosensing Techniques, Antigens, Viral, Nanoparticles, Immunoassay, Limit of Detection, Antibodies, Viral, Antibodies, Immobilized, COVID-19 Serological Testing, Polyethylene Glycols