New approaches for solubilization of phosphate rocks through solid-state fermentation by optimization of oxalic acid production.

Natalia Alvarez Rodrigues, Mariane Molina Buffo, Fernanda Perpétua Casciatori, Cristiane Sanchez Farinas

Journal: Bioresource technology 2024;408():131165

PMID: 39069142

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Abstract

This study explores the enhancement of phosphate rock (PR) solubilization through solid-state fermentation (SSF) by optimizing oxalic acid production using Aspergillus niger. Key process parameters, including the use of agro-industrial by-products (sugarcane bagasse (SCB), wheat bran (WB), soybean bran (SB)), pH levels, sucrose supplementation, and methanol addition, were systematically evaluated through sequential experimental designs. The results identified SCB and SB in a 1:1 ratio as the most effective substrate. Remarkably, the inclusion of methanol (7 %) and sucrose (0.5 %) resulted in a 3-fold increase in oxalic acid production. Under these optimized conditions, significant phosphorus solubilization of Bayóvar, Itafós, and Registro PRs was achieved, with Bayóvar rock releasing up to 12.1 g/kgds of soluble P (63.8 % efficiency). Additionally, the SSF process effectively released organic phosphorus from the agro-industrial substrates. These findings hold promise for advancing the bio-based economy and developing future industrial biofertilizers.

Address: Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís km 235 - SP-310, 13565-905 São Carlos, SP, Brazil; Embrapa Instrumentação, Rua XV de Novembro 1452, 13565-905 São Carlos, SP, Brazil; Chemical Engineering Department, Federal University of São Carlos, Rod. Washington Luís km 235 - SP-310, 13565-905 São Carlos, SP, Brazil.; Chemical Engineering Department, Federal University of São Carlos, Rod. Washington Luís km 235 - SP-310, 13565-905 São Carlos, SP, Brazil.; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís km 235 - SP-310, 13565-905 São Carlos, SP, Brazil; Chemical Engineering Department, Federal University of São Carlos, Rod. Washington Luís km 235 - SP-310, 13565-905 São Carlos, SP, Brazil.; Graduate Program of Chemical Engineering, Federal University of São Carlos, Rod. Washington Luís km 235 - SP-310, 13565-905 São Carlos, SP, Brazil; Embrapa Instrumentação, Rua XV de Novembro 1452, 13565-905 São Carlos, SP, Brazil. Electronic address: [email protected].

DOI: 10.1016/j.biortech.2024.131165

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MeSH Terms: Oxalic Acid, Fermentation, Solubility, Phosphates, Aspergillus niger, Hydrogen-Ion Concentration, Cellulose, Glycine max, Methanol, Sucrose, Saccharum