In situ detection of antigen-specific tumor-infiltrating lymphocytes using newly designed tetramers.

Rachel Bras-Gonçalves, Frédérique-Anne Le Gal, Marie-Françoise Avril, Marc Dalod, Francine Connan, Aude Chaboissier, Jeannine Choppin, Jean-Gérard Guillet

Journal: Journal of immunological methods 2003;280(1-2):103-11

PMID: 12972191

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Abstract

We described a new process for the design of HLA tetramers using soluble MHC class I molecules purified from Epstein Barr Virus-transformed B cells. This method does not rely on genetic engineering and presents a significant advantage in view of the polymorphism of MHC class I molecules because tetramers can be produced with any HLA molecule. Here, we showed that our HLA-A*0201 tetramers provided experimental results similar to those obtained with tetramers made with recombinant MHC molecules. Moreover, they can be used to efficiently identify peptide-specific T cells from ex vivo PBMCs as well as from lymphocytes infiltrating human tumor. This innovative and simple method could be widely adopted, specially in diagnostic procedures for monitoring peptide-based immunotherapy.

Address: Département d'Immunologie, Inserm U567,CNRS UMR 8104, Université Paris V René Descartes, IFR Alfred Jost, Institut Cochin, 27 rue du Faubourg Saint Jacques 75014 Paris, France.

DOI: 10.1016/s0022-1759(03)00266-7

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MeSH Terms: Amino Acid Sequence, Antigen Presentation, Cell Line, HIV Infections, HLA-A Antigens, HLA-A2 Antigen, Humans, Immunologic Techniques, In Vitro Techniques, Lymphocytes, Tumor-Infiltrating, Melanoma, Oligopeptides, Protein Structure, Quaternary, T-Lymphocytes