Selenium biomarkers and miR-7-5p in overweight/obese women.

Silvia Maria Franciscato Cozzolino, Bruna Zavarize Reis, Graziela Biude Silva Duarte, Marcelo Macedo Rogero, Fernando Barbosa, Higor Paiva de Mendonça Alves, Adriano Carlos de Souza Junior, Leonam da Silva Pereira Batista, Raquel Costa Silva Dantas-Komatsu, Karina Zaira Silva Marinho Costa

Journal: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) 2024;86():127499

PMID: 39084121

Full Text on Elsevier Science View on PubMed

Abstract

INTRODUCTION

Chronic low-grade inflammation and oxidative stress are pivotal contributors to the metabolic complications associated with obesity. Selenoprotein P (SELENOP) and glutathione peroxidase 1 (GPx1) are selenoproteins involved in the reduction of reactive oxygen species and pro-inflammatory cytokines levels. Nutritional epigenomics revealed the interaction of microRNAs and nutrients with an important impact on metabolic pathways involved in obesity. However, the knowledge regarding the influence of microRNA on selenium biomarkers and its impact on metabolic pathways related to obesity remains scarce. Thus, the aim of this study was to investigate the association of plasma miR-7-5p expression with selenium and inflammatory biomarkers in women with overweight/obesity.

MATERIAL AND METHODS

Anthropometric evaluations were performed and blood samples were collected for the analysis of fasting glucose, insulin, inflammatory and selenium biomarkers, and miR-7-5p expression in 54 women with overweight/obesity. Gene expression of SELENOP and GPX1 were evaluated in peripheral mononuclear blood cells.

RESULTS

This study observed a negative correlation between SELENOP levels and miR-7-5p (rho = -0.350; p = 0.018). Additionally, it was observed that body fat (OR = 0.737; p = 0.011), age (OR = 1.214; p = 0.007), and miR-7-5p (OR = 0.990; p = 0.015) emerged as significant predictors of SELENOP levels.

CONCLUSIONS

In conclusion, we observed a significant inverse association between miR-7-5p expression and SELENOP concentration in overweight/obese women, suggesting that age and percentage of body fat are also associated.

TRIAL REGISTRATION NUMBER

Brazilian Registry of Clinical Trials (ReBEC) number RBR-2nfy5q.

Address: Postgraduate Program in Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Rio Grande do Norte, Avenida Senador Salgado Filho, 3000. University Campus - Lagoa Nova, Natal, RN 59078-970, Brazil. Electronic address: [email protected].; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, Avenida Prof. Lineu Prestes, 580, Bloco 14 - Butantã, São Paulo, SP 05508-000, Brazil. Electronic address: [email protected].; Postgraduate Program in Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Rio Grande do Norte, Avenida Senador Salgado Filho, 3000. University Campus - Lagoa Nova, Natal, RN 59078-970, Brazil. Electronic address: [email protected].; Postgraduate Program in Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Rio Grande do Norte, Avenida Senador Salgado Filho, 3000. University Campus - Lagoa Nova, Natal, RN 59078-970, Brazil. Electronic address: [email protected].; Department of Nutrition, School of Public Health, University of São Paulo, Avenida Dr. Arnaldo, 715 - Cerqueira César, São Paulo, SP 01246-904, Brazil; Food Research Center (FoRC), CEPID-FAPESP, Research Innovation and Dissemination Centers São Paulo Research Foundation, Laboratory of Food Engineering, Semi Industrial Ed. - R. do Lago, 250 - Bloco C, São Paulo, SP 05468-140, Brazil. Electronic address: [email protected].; Department of Clinical, Toxicological and Bromatological Analysis, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/nº - Vila Monte Alegre, Ribeirão Preto, SP 14040903, Brazil. Electronic address: [email protected].; Department of Food and Experimental Nutrition, Faculty of Pharmaceutical Science, University of São Paulo, Avenida Prof. Lineu Prestes, 580, Bloco 14 - Butantã, São Paulo, SP 05508-000, Brazil. Electronic address: [email protected].; Postgraduate Program in Pharmaceutical Sciences, Center for Health Sciences, Federal University of Rio Grande do Norte, Rio Grande do Norte, Rua General Gustavo Cordeiro de Faria, s/nº - Petrópolis, Natal, RN 59012-570, Brazil. Electronic address: [email protected].; Brazilian Company of Hospital Services (EBSERH), Onofre Lopes University Hospital, Av. Nilo Peçanha, 620 - Petrópolis, Natal, RN 59012-300, Brazil. Electronic address: [email protected].; Postgraduate Program in Nutrition, Center for Health Sciences, Federal University of Rio Grande do Norte, Rio Grande do Norte, Avenida Senador Salgado Filho, 3000. University Campus - Lagoa Nova, Natal, RN 59078-970, Brazil; Department of Nutrition, Federal University of Rio Grande do Norte, Rio Grande do Norte, Avenida Senador Salgado Filho, 3000, University Campus - Lagoa Nova, Natal, RN 59078-970, Brazil. Electronic address: [email protected].

DOI: 10.1016/j.jtemb.2024.127499

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MeSH Terms: Humans, Female, MicroRNAs, Selenium, Obesity, Biomarkers, Adult, Overweight, Middle Aged, Glutathione Peroxidase GPX1, Selenoprotein P