The prevalence of controls in phyllosphere microbiome research: a methodological review.

Brady L Welsh, Raphael Eisenhofer

Journal: The New phytologist 2024;242(1):23-29

PMID: 38339825

Abstract

DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.

Address: School of Biological Sciences, The University of Adelaide, North Terrace Campus, Adelaide, SA, 5005, Australia.; School of Biological Sciences, The University of Adelaide, North Terrace Campus, Adelaide, SA, 5005, Australia.; Center for Evolutionary Hologenomics, Globe Institute, University of Copenhagen, Copenhagen, 1353, Denmark.

DOI: 10.1111/nph.19573

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Full Text Sources:

https://onlinelibrary.wiley.com/doi/10.1111/nph.19...

Miscellaneous:

https://assays.cancer.gov/CPTAC-68

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-HLA-B-1

MeSH Terms: Prevalence, Systematic Reviews as Topic, Microbiota