The mechanism of intrinsic peroxidase (POD)-like activity of attapulgite.

Peixia Wang, Yihe Zhang, Qi An, Xiao Zhang, Feng Feng, Bin Mu

Journal: Analytical and bioanalytical chemistry 2024;416(27):6033-6044

PMID: 38602542

Abstract

Natural attapulgite (ATP) is a promising substitute for existing artificial nanozymes due to its intrinsic enzymatic activity. However, the active center of ATP's inherent enzymatic activity has not yet been revealed, which limits its further design and activity optimization. Studying the active center of mineral materials can be extremely challenging due to their complexity. Here, we demonstrated that Fe is the primary element in ATP responsible for peroxidase (POD)-like activity through theoretical speculation and experimental verification. More importantly, we found that the ratio of Fe/Fe is responsible for the district POD-like activity of ATP from different regions with the same Fe content. Additionally, three facile strategies, including grinding, heat treatment, and acid treatment, were demonstrated to increase the relative Fe content and thus optimize the POD-like activity of ATP. Finally, ATP was used to detect the concentration of HO, enabling the detection of low concentrations (0.11-1.76 mM) of HO. This study serves as a novel reference for the future design and performance optimization of nanozymes that are based on ATP and clay minerals.

Address: Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources, Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing, 100083, China.; Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources, Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing, 100083, China. [email protected].; Key Laboratory of Clay Mineral Applied Research of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China.; Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources, Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes, National Laboratory of Mineral Materials, School of Materials Science and Technology, China University of Geosciences (Beijing), Beijing, 100083, China. [email protected].; National Anti-Drug Laboratory Beijing Regional Center, Beijing, 100164, China. [email protected].; Beijing Narcotics Control Technology Center, Beijing, 100164, China. [email protected].

DOI: 10.1007/s00216-024-05280-2

Link outs

Subscription / membership required

Full Text Sources:

https://dx.doi.org/10.1007/s00216-024-05280-2

MeSH Terms: Hydrogen Peroxide, Magnesium Compounds, Silicon Compounds, Peroxidase, Iron