Amyloplast is involved in the MIZ1-modulated root hydrotropism.

Yadi Chen, Siqi Liu, Lulu Wang, Weifeng Xu, Jianhua Zhang, Ying Li, Yonghui Hong, Zixuan Yao, Shuqiu Jiang, Xinkai Zhu

Journal: Journal of plant physiology 2024;296():154224

PMID: 38507925

Full Text on Elsevier Science View on PubMed

Abstract

Roots exhibit hydrotropism in response to moisture gradients, with the hydrotropism-related gene Mizu-kussei1 (MIZ1) playing a role in regulating root hydrotropism in an oblique orientation. However, the mechanisms underlying MIZ1-regulated root hydrotropism are not well understood. In this study, we employed obliquely oriented experimental systems to investigate root hydrotropism in Arabidopsis. We found that the miz1 mutant displays reduced root hydrotropism but increased root gravitropism following hydrostimulation, as compared to wild-type plants. Conversely, overexpression of AtMIZ1 leads to enhanced root hydrotropism but decreased root gravitropism following hydrostimulation, as compared to wild-type plants. Using co-immunoprecipitation followed by mass spectrometry (IP-MS), we explored proteins that interact with AtMIZ1, and we identified PGMC1 co-immunoprecipitated with MIZ1 in vivo. Furthermore, the miz1 mutant exhibited higher expression of the PGMC1 gene and increased phosphoglucomutase (PGM) activity, while AtMIZ1 overexpressors resulted in lower expression of the PGMC1 gene, reduced amyloplast amount, and reduced PGM activity in comparison to wild-type roots. In addition, different Arabidopsis natural accessions having difference in their hydrotropic response demonstrated expression level of PGMC1 was negatively correlated with hydrotropic root curvature and AtMIZ1 expression. Our results provide valuable insights into the role of amyloplast in MIZ1-regulated root hydrotropism.

Address: Jiangsu Key Laboratory of Crop Genomics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, 225009, China.; College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, 225009, China.; Jiangsu Key Laboratory of Crop Genomics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, 225009, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China.; Joint International Research Laboratory of Water and Nutrient in Crops, Center for Plant Water-Use and Nutrition Regulation and College of Resource and Environment, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.; Department of Biology, Hong Kong Baptist University, Hong Kong, 999077, China; School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, 999077, China. Electronic address: [email protected].; Jiangsu Key Laboratory of Crop Genomics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology, Agricultural College of Yangzhou University, Yangzhou, 225009, China; Department of Biology, Hong Kong Baptist University, Hong Kong, 999077, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009, China; Joint International Research Laboratory of Water and Nutrient in Crops, Center for Plant Water-Use and Nutrition Regulation and College of Resource and Environment, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China. Electronic address: [email protected].

DOI: 10.1016/j.jplph.2024.154224

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MeSH Terms: Arabidopsis, Arabidopsis Proteins, Water, Tropism, Gravitropism, Plant Roots