Characteristic analysis and fermentation optimization of a novel Aureobasidium pullulans RM1603 with high pullulan yield.

Hongxin Zhao, Shuting Zhang, Ye Lu, Fangxu Xu, Li Liu, Jiale Chen, Ruoxuan Bai, Yaqiao Hao, Buyun Wei

Journal: Journal of bioscience and bioengineering 2024;137(5):335-343

PMID: 38413318

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Abstract

A high-yielding microbial polysaccharide-producing strain, named RM1603, was isolated from rhizosphere soil and identified by morphological and phylogenetic analysis. The extracellular polysaccharides (EPS) were identified by thin-layer chromatography and infrared spectroscopy. The fermentation conditions were optimized by single factor experiments in shake flasks and a 5-L fermentor. The results of morphological and phylogenetic tree analysis showed that RM1603 was a strain of Aureobasidium pullulans. Its microbial polysaccharide was identified as pullulan, and the EPS production capacity reached 33.07 ± 1.03 g L in shake flasks. The fermentation conditions were optimized in a 5-L fermentor, and were found to encompass an initial pH of 6.5, aeration rate of 2 vvm, rotor speed of 600 rpm, and inoculum size of 2 %. Under these conditions, the pullulan yield of RM1603 reached 62.52 ± 0.24 g L. Thus, this study contributes RM1603 as a new isolation with high-yielding pullulan and potential application value in biotechnology.

Address: Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.; The Research Institute for Cordyceps Militaris with Functional Value of Industrial Technology Research Academy of Liaoning Province, Shenyang 110034, China.; The Research Institute for Cordyceps Militaris with Functional Value of Industrial Technology Research Academy of Liaoning Province, Shenyang 110034, China; Liaoning Province Key Laboratory of Cordyceps Militaris with Functional Value, Experimental Teaching Center, Shenyang Normal University, Shenyang 110034, China.; Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; Liaoning Province Key Laboratory of Cordyceps Militaris with Functional Value, Experimental Teaching Center, Shenyang Normal University, Shenyang 110034, China. Electronic address: [email protected].

DOI: 10.1016/j.jbiosc.2023.12.018

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MeSH Terms: Fermentation, Phylogeny, Ascomycota, Polysaccharides, Aureobasidium, Glucans