A novel colorimetric sensor composed from alkaline phosphatase-graphene oxide nanoconjugates and enzyme induced gold nanoparticle aggregation for the detection of deoxynivalenol-3-glucoside.

Haiyan Fu, Peter Horvatovich, Minjie Zhao, Eric Marchioni, Yi Liu, Li Zhou, Yixin Suo, Yuanbin She

Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2025;341():126433

PMID: 40412232

Full Text on Elsevier Science View on PubMed

Abstract

Grains and goji berry are highly susceptible to fungal and mycotoxin during the processing and storage. Current detection methods require the use of complex sample preparation, expensive analytical platforms and professional operators, making rapid on-site detection difficult. This study explored a sensor based on β-Co(OH) nanosheets mediated composite material from graphene oxide (MGO), polyethyleneimine (PEI), and alkaline phosphatase (ALP) referred as MGO@PEI-ALP conjugate for the detection of masked mycotoxin (Deoxynivalenol-3-glucoside, D3G) in goji berries. The new sensor was able to detect D3G in the range of 0.50-3.50 mg/L with a 0.30 mg/L limit of detection, well below the maximum standard limit (1.0 mg) specified by the European Union. Meanwhile, this method could effectively remove D3G from complex dietary agricultural products. This study suggested a rapid visualization approach for the recognition of masked mycotoxin, offering valuable insights for food safety and quality.

Address: National Demonstration Center for Experimental Ethnopharmacology Education, School of Pharmaceutical Sciences, South-Central MinZu University, Wuhan 430074, PR China.; Department of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, A. Deusinglaan 1, 9713AV Groningen, The Netherlands. Electronic address: [email protected].; Equipe de Chimie Analytique des Molécules Bioactives et Pharmacognosie, Institut Pluridisciplinaire Hubert Curien (UMR 7178, CNRS/UDS), 74 route du Rhin, 67400 Illkirch, France.; College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China. Electronic address: [email protected].; National Demonstration Center for Experimental Ethnopharmacology Education, School of Pharmaceutical Sciences, South-Central MinZu University, Wuhan 430074, PR China. Electronic address: [email protected].

DOI: 10.1016/j.saa.2025.126433

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MeSH Terms: Graphite, Trichothecenes, Alkaline Phosphatase, Gold, Metal Nanoparticles, Colorimetry, Glucosides, Limit of Detection, Food Contamination