Protocol for the decellularization of human intestinal tissue from patients with inflammatory bowel disease.

Ren Mao, Pranab K Mukherjee, Florian Rieder, Sinan Lin, Jie Wang, Gail West, Ankita Prasad, Suhanti Banerjee

Journal: STAR protocols 2025;6(2):103829

PMID: 40408251

Free Full Text on Elsevier Science View on PubMed

Abstract

Decellularization of the human intestine in patients with inflammatory bowel disease (IBD) provides an experimental platform for the investigation of the bowel extracellular matrix (ECM). Here, we present a protocol for removing cellular components from the IBD intestinal tissue while preserving its structure and ECM components. We describe steps for pre-processing specimens and decellularization by osmotic pressure difference, followed by detergents and enzymes. We then detail procedures for extensive washing. The decellularized tissue can be stored or processed according to the experimental requirements. For complete details on the use and execution of this protocol, please refer to Lin et al..

Address: Department of Gastroenterology and Hepatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Center for Global Translational Inflammatory Bowel Disease Research, Cleveland Clinic, Cleveland, OH, USA; Department of Gastroenterology, Hepatology and Nutrition, Digestive Disease Institute, Cleveland Clinic, Cleveland, OH, USA.; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Henan Key Laboratory of Immunology and Targeted Drug, Xinxiang Medical University, Xinxiang, Henan Province, China.; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA.; Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA; Center for Global Translational Inflammatory Bowel Disease Research, Cleveland Clinic, Cleveland, OH, USA; Department of Gastroenterology, Hepatology and Nutrition, Digestive Disease Institute, Cleveland Clinic, Cleveland, OH, USA. Electronic address: [email protected].

DOI: 10.1016/j.xpro.2025.103829

Link outs

Free resources

Full Text Sources:

MeSH Terms: Humans, Inflammatory Bowel Diseases, Intestines, Decellularized Extracellular Matrix, Extracellular Matrix