Novel bioreduction system for the production of chiral alcohols.

M Kataoka, K Kita, M Wada, Y Yasohara, J Hasegawa, S Shimizu

Journal: Applied microbiology and biotechnology 2004;62(5-6):437-45

PMID: 12838375

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Abstract

Chiral alcohols are useful intermediates for many pharmaceuticals and chemicals. Enzymatic asymmetric reduction of prochiral carbonyl compounds is a promising method for producing chiral alcohols. There have been many attempts to construct bioreduction systems for the industrial production of chiral alcohols. This review focuses on the establishment of a novel bioreduction system using an Escherichia coli transformant co-expressing genes for carbonyl reductase and cofactor-regeneration enzyme. This bioreduction system could be useful as an all-purpose catalyst for asymmetric reduction reactions.

Address: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502 Kyoto, Japan.

DOI: 10.1007/s00253-003-1347-y

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MeSH Terms: Alcohol Oxidoreductases, Alcohols, Aldehyde Reductase, Aldo-Keto Reductases, Bacillus megaterium, Basidiomycota, Candida, Cloning, Molecular, Coenzymes, Escherichia coli, Fatty Acid Synthases, Glucose 1-Dehydrogenase, NADH, NADPH Oxidoreductases, Oxidation-Reduction, Recombinant Proteins, Stereoisomerism