Victoria Fuentes, Alessandra Reis, Laura Ceballos, Alessandro D Loguercio, Michael Willian Favoreto, Laryssa Mylenna Madruga Barbosa, Daniel Jiménez-Díez
Journal: Journal of dentistry 2025;160():105860
PMID: 40446901
OBJECTIVES
To evaluate hydrogen peroxide (HP) penetration into teeth with enamel cracks and to assess the effectiveness of a gingival barrier as a protective strategy. Additionally, the study examined whether the use of a gingival barrier affects bleaching efficacy.
MATERIALS AND METHODS
Thirty human premolars (n = 10 per group) were allocated into three experimental groups: intact teeth, teeth with enamel cracks, and teeth with enamel cracks protected with a gingival barrier. Cracks were detected using transillumination and measured using ImageJ software. In the third group, the gingival barrier was applied directly over the cracks, and the buccal surface of all specimens was also isolated with a gingival barrier (6 mm radius) and light-cured for 30 s. Bleaching was performed with 35 % hydrogen peroxide in two 30-minute sessions, with a seven-day interval apart. HP penetration into the pulp chamber was quantified using UV-Vis spectrophotometry. Bleaching efficacy was assessed by digital spectrophotometry (ΔE and ΔWI). Data were analyzed using one-way and two-way ANOVA (α = 0.05).
RESULTS
Teeth exhibited vertical cracks averaging 4.2 ± 1.3 mm in length. Cracked teeth demonstrated 35 % higher HP penetration compared to intact teeth (p < 0.05). Covering the cracks with a gingival barrier did not reduce HP penetration (p = 0.22). No significant differences were found in bleaching efficacy among the groups (p > 0.05).
CONCLUSIONS
Enamel cracks significantly increased HP penetration. The gingival barrier did not reduce diffusion but did not compromise bleaching efficacy.
CLINICAL RELEVANCE
Teeth presenting enamel cracks showed significantly increased hydrogen peroxide penetration. Alternative protective strategies should be explored, as gingival barriers alone appear insufficient to effectively seal enamel cracks.
Copyright © 2025 Elsevier Ltd. All rights reserved.
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