Structural characterization and biotechnological potential of an exopolysaccharide produced by Bacillus licheniformis F2LB isolated from Fumarole Bay of Deception Island, Antarctica.

Aparna Banerjee, João Paulo Fabi, Catherine Ormeño, Srijan Banerjee, Nicolás Flores-Castañón, Francisca Valenzuela, Shrabana Sarkar, Alexander Galán, Dipayan Samanta, Priya Saxena, Rajesh K Sani, Manik Prabhu Narsing Rao, Gustavo Cabrera-Barjas

Journal: International journal of biological macromolecules 2025;321(Pt 1):146114

PMID: 40683489

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Abstract

Deception Island, Antarctica, harbors unique polyextreme conditions fostering versatile microorganisms with significant biotechnological potential. This is due to genomic adaptation to extreme conditions that lead to metabolic alterations, such as the production of unique exopolysaccharides. Bacillus licheniformis F2LB, isolated from Deception Island, Antarctica, was evaluated for its exopolysaccharide's structural and biotechnological potential (EPS). Genome analysis revealed EPS biosynthesis, UV resistance, and thermal adaptation genes. The EPS with a putative glucogalactomannan backbone, comprised mainly of a sugar molar ratio Man: Glu: Xyl: Fuc (1: 0.7: 0.4: 0.3) and other sugars in trace amounts. Spectroscopy identified key glycosidic linkages, and morphology showed a rough, nonporous surface. Functionally, EPS demonstrated robust thermal stability (T 255 °C), remarkable antioxidant activities (~100 % H₂O₂ scavenging; DPPH: 75.5 %), good emulsifying ability (90 % in olive oil), and water (238.7 %) and oil-holding capacities (384.7 %) exceeding commercial xanthan gum. These findings underline its potential as a sustainable food additive.

Address: Functional Polysaccharides Research Group, Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, Talca, Chile. Electronic address: [email protected].; Department of Food Science and Experimental Nutrition, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil; Food Research Center (FoRC), CePID-FAPESP (Research, Innovation and Dissemination Centers, São Paulo Research Foundation), CEPIX, São Paulo, SP, Brazil.; Functional Polysaccharides Research Group, Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, Talca, Chile.; PhD Program in Science, R&D Bioactive Products Department, Chemistry Institute of Natural Resources, University of Talca, Talca, Chile.; Doctorado en Ciencias Biomédicas, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Talca, Chile.; Centro de Investigación de Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Talca, Chile; Centro Regional de Estudios Ambientales (CREA), Universidad Católica de la Santísima Concepción, Concepción, Chile.; Karen M. Swindler Department of Chemical and Biological Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.; Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, Talca 3460000, Chile.; Facultad de Ciencias de la Rehabilitación y Calidad de Vida, Universidad San Sebastián, Campus Las Tres Pascualas, Lientur 1457, 4080871 Concepción, Chile.

DOI: 10.1016/j.ijbiomac.2025.146114

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MeSH Terms: Polysaccharides, Bacterial, Bacillus licheniformis, Antarctic Regions, Biotechnology, Antioxidants