Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training.

A S Deshmukh, D E Steenberg, M Hostrup, J B Birk, J K Larsen, A Santos, R Kjøbsted, J R Hingst, C C Schéele, M Murgia, B Kiens, E A Richter, M Mann, J F P Wojtaszewski

Journal: Nature communications 2021;12(1):304

PMID: 33436631

Free Full Text on Nature Publishing Group View on PubMed

Abstract

Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca signaling, and fat and glucose metabolism adapts to training in a fiber type-specific manner. Our data provide a resource to elucidate molecular mechanisms underlying muscle function and health, and our workflow allows fiber type-specific proteomic analyses of snap-frozen non-embedded human muscle biopsies.

Address: The Novo Nordisk Foundation Center for Protein Research, Clinical Proteomics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. [email protected].; The Novo Nordisk Foundation Center for Basic Metablic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. [email protected].; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.; Section of Integrative Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark.; The Novo Nordisk Foundation Center for Basic Metablic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.; The Novo Nordisk Foundation Center for Protein Research, Clinical Proteomics, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.; The Centre of Inflammation and Metabolism and Centre for Physical Activity Research Rigshospitalet, University Hospital of Copenhagen, Copenhagen, Denmark.; Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.; Department of Biomedical Sciences, University of Padua, Padua, Italy.; Section of Molecular Physiology, Department of Nutrition, Exercise and Sports, University of Copenhagen, Copenhagen, Denmark. [email protected].

DOI: 10.1038/s41467-020-20556-8

Link outs

Free resources

Full Text Sources:

Medical:

Other Literature Sources:

https://scite.ai/reports/33436631

Miscellaneous:

https://assays.cancer.gov/CPTAC-177

Molecular Biology Databases:

https://www.informatics.jax.org/reference/33436631

Subscription / membership required

Other Literature Sources:

https://facultyopinions.com/pubmed/33436631

MeSH Terms: Adaptation, Physiological, Biomarkers, Biopsy, Exercise, Freeze Drying, Glucose, Humans, Mitochondria, Muscle Fibers, Fast-Twitch, Muscle Fibers, Skeletal, Muscle Fibers, Slow-Twitch, Muscle, Skeletal, Principal Component Analysis, Proteome, Proteomics