An efficient capture strategy for the purification of human adenovirus type 5 from cell lysates.

Sandra Dietl, Franziska Kiefer, Stefanie Binder, Paul Walther, Harald Sobek, Boris Mizaikoff

Journal: Journal of biotechnology 2022;361():49-56

PMID: 36494010

Full Text on Elsevier Science View on PubMed

Abstract

An efficient capture step for human adenovirus type 5 from cell lysate was developed as an initial virus purification step from cell debris supernatant. Organosilane-based polymer particles were synthesized and experimental monomer screening allowed the selection of appropriate functionalities for the development of particles for virus binding. After elution, virus recoveries of 83 % were obtained with significant reduction of matrix proteins and residual host cell DNA. Therefore, the implemented capture strategy for adenovirus via polymer particles provides a scalable and reproducible approach to reduce time and cost during virus purification processes.

Address: Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.; Boehringer Ingelheim Therapeutics GmbH, Beim Braunland 1, 88416 Ochsenhausen, Germany.; Zentrale Einrichtung Elektronenmikroskopie, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany.; Dinglingerstrasse 10/3, 88400 Biberach, Germany.; Institute of Analytical and Bioanalytical Chemistry, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany; Hahn-Schickard, Sedanstrasse 14, 89077 Ulm, Germany. Electronic address: [email protected].

DOI: 10.1016/j.jbiotec.2022.11.015

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MeSH Terms: Humans, Adenoviruses, Human, Adenoviridae, Polymers, DNA, Proteins