The effect of Holder pasteurization on the lipid and metabolite composition of human milk.

Isabel Ten-Doménech, Victoria Ramos-Garcia, Marta Moreno-Torres, Anna Parra-Llorca, María Gormaz, Máximo Vento, Julia Kuligowski, Guillermo Quintás

Journal: Food chemistry 2022;384():132581

PMID: 35257998

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Abstract

Human milk (HM) is the gold standard for newborn nutrition. When own mother's milk is not sufficiently available, pasteurized donor human milk becomes a valuable alternative. In this study we analyzed the impact of Holder pasteurization (HoP) on the metabolic and lipidomic composition of HM. Metabolomic and lipidomic profiles of twelve paired HM samples were analysed before and after HoP by liquid chromatography-mass spectrometry (MS) and gas chromatography-MS. Lipidomic analysis enabled the annotation of 786 features in HM out of which 289 were significantly altered upon pasteurization. Fatty acid analysis showed a significant decrease of 22 out of 29 detectable fatty acids. The observed changes were associated to five metabolic pathways. Lipid ontology enrichment analysis provided insight into the effect of pasteurization on physical and chemical properties, cellular components, and functions. Future research should focus on nutritional and/or developmental consequences of these changes.

Address: Neonatal Research Group, Health Research Institute Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain.; Unidad de Hepatología Experimental, Health Research Institute Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain.; Neonatal Research Group, Health Research Institute Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain; Division of Neonatology, University & Polytechnic Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain.; Neonatal Research Group, Health Research Institute Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain. Electronic address: [email protected].; Health and Biomedicine, Leitat Technological Center, Carrer de la Innovació 2, 08225 Terrassa, Spain; Analytical Unit, Health Research Institute La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain.

DOI: 10.1016/j.foodchem.2022.132581

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MeSH Terms: Humans, Infant, Newborn, Lipids, Mass Spectrometry, Milk, Human, Pasteurization