Impact of Curcumin on Hepatic Low-Density Lipoprotein Uptake.

Mohammad Jalili-Nik, Khadijeh Mahboobnia, Paul C Guest, Muhammed Majeed, Khalid Al-Rasadi, Tannaz Jamialahmadi, Amirhossein Sahebkar

Journal: Methods in molecular biology (Clifton, N.J.) 2022;2343():395-400

PMID: 34473340

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Abstract

Elevated levels of plasma low-density lipoprotein cholesterol (LDL-C) are causally related to atherosclerotic cardiovascular disease. Enhancing the removal of LDL particles from the plasma, mainly by the liver, is the most efficient strategy for reducing LDL-C and the ensuing atherosclerosis. In this context, polyphenolic compounds like curcumin have generated interest owing to their lipid-modifying capacity. The promising effect of curcumin has been studied in attenuating atherosclerosis (in experimental models), and correcting dyslipidemia (in clinical studies). The underlying mechanisms of the effects of curcumin are relatively unknown, and the impact of curcumin on hepatic LDL uptake warrants further investigations. Here, we present a protocol to assess the effects of curcumin on LDL uptake in hepatocytes.

Address: Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Department of Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.; Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil.; Sabinsa Corporation, East Windsor, NJ, USA.; Medical Research Centre, Sultan Qaboos University, Muscat, Oman.; Department of Food Science and Technology, Quchan Branch, Islamic Azad University, Quchan, Iran.; Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. [email protected].; Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran. [email protected].; School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. [email protected].; Polish Mother's Memorial Hospital Research Institute (PMMHRI), Lodz, Poland. [email protected].

DOI: 10.1007/978-1-0716-1558-4_29

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https://doi.org/10.1007/978-1-0716-1558-4_29

MeSH Terms: Atherosclerosis, Cholesterol, LDL, Curcumin, Hepatocytes, Humans, Liver