Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of γ-FeO nanoparticles.

Yudum Tepeli Büyüksünetçi, Burak Ekrem Çitil, Utku Tapan, Ülkü Anık

Journal: Mikrochimica acta 2021;188(10):335

PMID: 34505191

Free Full Text on PubMed Central View on PubMed

Abstract

A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ FeO nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ FeO nanoparticles, the color change of HO included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained.

Address: Faculty of Science, Chemistry Department, Mugla Sitki Kocman University, 48000, Kotekli, Mugla, Turkey.; Faculty of Medicine, Department of Medical Microbiology, Mugla Sitki Kocman University, Kotekli, Mugla, Turkey, 4800.; Faculty of Medicine, Department of Chest Diseases, Mugla Sitki Kocman University, Kotekli, Mugla, Turkey, 4800.; Faculty of Science, Chemistry Department, Mugla Sitki Kocman University, 48000, Kotekli, Mugla, Turkey. [email protected].

DOI: 10.1007/s00604-021-04989-6

Link outs

Free resources

Full Text Sources:

Medical:

Miscellaneous:

https://assays.cancer.gov/CPTAC-438

MeSH Terms: Angiotensin-Converting Enzyme 2, Benzidines, Biosensing Techniques, COVID-19, COVID-19 Testing, Catalysis, Chromogenic Compounds, Colorimetry, Cysteine, Humans, Hydrogen Peroxide, Limit of Detection, Magnetic Iron Oxide Nanoparticles, Nasopharynx, Oropharynx, Oxidation-Reduction, Peroxidase, SARS-CoV-2, Spike Glycoprotein, Coronavirus