EPR Spectroscopy as a Method for ROS Quantification in the Skin.

Silke B Lohan, Daniela Ivanov, Nadine Schüler, Bastian Berger, Stephanie Albrecht, Martina C Meinke

Journal: Methods in molecular biology (Clifton, N.J.) 2021;2202():137-148

PMID: 32857353

Abstract

Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.

Address: Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany.; Freiberg Instruments GmbH, Freiberg, Germany.; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany. [email protected].

DOI: 10.1007/978-1-0716-0896-8_12

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MeSH Terms: Cyclic N-Oxides, Electron Spin Resonance Spectroscopy, Free Radicals, Humans, Reactive Oxygen Species, Skin, Spin Labels, Ultraviolet Rays