FTIR spectroscopy as a novel analytical approach for investigation of glucose transport and glucose transport inhibition studies in transwell in vitro barrier models.

Mario Rothbauer, Christoph Eilenberger, Sarah Spitz, Barbara Bachmann, Jasmin Pajenda, Andreas Schwaighofer, Gregor Höll, Palle Steen Helmke, Yvonne Kohl, Bernhard Lendl, Peter Ertl

Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021;237():118388

PMID: 32361318

Full Text on Elsevier Science View on PubMed

Abstract

Glucose transport is key for cellular metabolism as well as physiological function and is maintained via passive facilitated and active sodium-glucose linked transport routes. Here, we present for the first time Fourier-transform infrared spectroscopy as a novel approach for quantification of apical-to-basolateral glucose transport of in vitro cell barrier models using liver, lung, intestinal and placental cancer cell lines. Results of our comparative study revealed that distinct differences could be observed upon subjection to transport inhibitors.

Address: Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria. Electronic address: [email protected].; Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.; Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; AUVA Research Centre, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, 1200 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.; Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria.; Fraunhofer Institute for Biomedical Engineering, 66280 Sulzbach, Germany.; Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Institute of Applied Synthetic Chemistry, Vienna University of Technology, Getreidemarkt 9, 1060 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria. Electronic address: [email protected].

DOI: 10.1016/j.saa.2020.118388

Link outs

Subscription / membership required

Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S1386...

MeSH Terms: Biological Transport, Caco-2 Cells, Cytochalasin B, Electric Impedance, Female, Glucose, HT29 Cells, Hep G2 Cells, Humans, Phloretin, Pregnancy, Proof of Concept Study, Spectroscopy, Fourier Transform Infrared, Trophoblasts, Tumor Cells, Cultured