Nickel-phosphate pompon flowers nanostructured network enables the sensitive detection of microRNA.

Palanisamy Kannan, Thandavarayan Maiyalagan, Bingyong Lin, Wang Lei, Chen Jie, Longhua Guo, Zhongqing Jiang, Shun Mao, Palaniappan Subramanian

Journal: Talanta 2020;209():120511

PMID: 31892041

Full Text on Elsevier Science View on PubMed

Abstract

An electrochemical immuno-nanogenosensor is developed based on noble-metal-free nickel phosphate nanostructure (NiPNs) as an excellent biocompatible material for miRNA detection in blood serum and urine samples without using indicators for the first time. The pompon flower-like morphology of NiPNs is synthesized, and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction pattern (XRD), fourier transform-infrared spectroscopy (FT-IR), and electrochemical impedance methods. The novel NiPNs nanostructured interface was constructed by coordinate covalent bonding between Ni and phosphate group of probe DNA. The constructed NiPNs-p-DNA surface served as the amplified hybridization platform enabling efficient access to numerous target microRNA sequences. As a result, the developed NiPFNs biosensing platform displayed excellent sensitivity, selectivity, and ultralow experimental limit-of-detection (LOD) of 0.034 pM (S/N = 3) as compared with other Ni phosphide nanostructures. This simple and efficient approach is highly suitable for the development of point-of-care detection systems. To the extent of our knowledge, this is the first report on trace level detection of miRNAs employing non-noble Ni metal nanostructures based biosensing platform.

Address: College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China. Electronic address: [email protected].; Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur, Chennai, 603203, Tamil Nadu, India.; Institute of Nanomedicine and Nanobiosensing, MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350116, PR China.; College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China.; College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China. Electronic address: [email protected].; Key Laboratory of Optical Field Manipulation of Zhejiang Province, Department of Physics, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.; State Key Laboratory of Pollution Control and Resource Reuse, International Joint Research Center for Sustainable Urban Water System, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai, 200092, PR China.; Department of Material Engineering, KU Leuven, Kasteelpark Arenberg 44, P.O. Box 2450, B-3001, Heverlee, Belgium.

DOI: 10.1016/j.talanta.2019.120511

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MeSH Terms: Biosensing Techniques, DNA, DNA Probes, Dielectric Spectroscopy, Limit of Detection, MicroRNAs, Nanostructures, Nickel, Nucleic Acid Hybridization, Phosphates