Epigenome-wide effects of vitamin D on asthma bronchial epithelial cells.

Nadia Boutaoui, Sandeep Puranik, Rong Zhang, Ting Wang, Daniel H Hui, John Brehm, Erick Forno, Wei Chen, Juan C Celedón

Journal: Epigenetics 2020;14(9):844-849

PMID: 31122150

Abstract

Vitamin D is a nutrient and a hormone with multiple effects on immune regulation and respiratory viral infections, which can worsen asthma and lead to severe asthma exacerbations. We set up a complete experimental and analytical pipeline for ATAC-Seq and RNA-Seq to study genome-wide epigenetic changes in human bronchial epithelial cells of asthmatic subjects, following treatment of these cells with calcitriol (vitamin D3) and Poly (I:C)(a viral analogue). This approach led to the identification of biologically plausible candidate genes for viral infections and asthma, such as DUSP10 and SLC44A1.

Address: a Division of Pediatric Pulmonary Medicine, UPMC Children's Hospital of Pittsburgh, University of Pittsburgh , Pittsburgh , PA , USA.; b Department of Statistics, University of Pittsburgh , Pittsburgh , PA , USA.; c Fred Hutchinson Cancer Research Center , Seattle , WA , USA.; d Brigham and Women's Hospital, Harvard Medical School, Broad Institute , Boston , MA , USA.; e Division of Pulmonary and Critical Care Medicine, University of Pittsburgh Medical Center- St. Margret's Hospital, University of Pittsburgh , Pittsburgh , PA , USA.

DOI: 10.1080/15592294.2019.1622993

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MeSH Terms: Antigens, CD, Asthma, Bronchi, Cells, Cultured, Dual-Specificity Phosphatases, Epigenesis, Genetic, Epigenomics, Epithelial Cells, Gene Expression Regulation, Humans, Mitogen-Activated Protein Kinase Phosphatases, Organic Cation Transport Proteins, Poly I-C, Sequence Analysis, RNA, Vitamin D