CpG island methylation of tumor-related genes in three primary central nervous system lymphomas in immunocompetent patients.

Pilar Gonzalez-Gomez, M Josefa Bello, Dolores Arjona, M Eva Alonso, Jesus Lomas, Cinthia Amiñoso, Jose M de Campos, Jose L Sarasa, Manuel Gutierrez, Juan A Rey

Journal: Cancer genetics and cytogenetics 2003;142(1):21-4

PMID: 12660028

Full Text on Elsevier Science View on PubMed

Abstract

We have determined the promoter CpG island methylation status of O(6)-methylguanine-DNA methyltransferase (MGMT), glutathione-S-transferase P1 (GSTP1), death-associated protein kinase (DAPK), p14(ARF), thrombospondin-1 (THBS1), tissue inhibitor of metalloproteinase-3 gene (TIMP-3), p73, p16(INK4A), RB1, and TP53 genes in three primary central nervous system lymphomas (PCNSL). Five genes (GSTP1, DAPK, TIMP-3, p16(INK4A), and RB1) were hypermethylated in two samples, whereas MGMT, THBS1, and p73 were aberrantly methylated in only one sample. No case presented CpG island methylation for the p14(ARF) and TP53 genes. These findings concur with previous data suggesting a frequent inactivation of p16(INK4A) and very limited involvement of TP53 in PCNSL and also provide insights into the epigenetic molecular involvement of other tumor-related genes in this neoplasm.

Address: Department of Experimental Surgery, Laboratorio de Oncogenetica Molecular, Hospital Universitario La Paz, Paseo Castellana 261, 28046 Madrid, Spain.

DOI: 10.1016/s0165-4608(02)00799-9