Expression of nuclear retinoic acid receptor in peripheral blood mononuclear cells (PBMC) of healthy subjects.

L Szabova, D Macejova, M Dvorcakova, S Mostbock, S Blazickova, S Zorad, S Walrand, N Cardinault, M P Vasson, E Rock, J Brtko

Journal: Life sciences 2003;72(7):831-6

PMID: 12479981

Full Text on Elsevier Science View on PubMed

Abstract

In vertebrates, both nuclear all-trans and 9-cis retinoic acid receptors (RAR and RXR) belonging to the steroid/thyroid/retinoid nuclear receptor superfamily play a crucial role in the vitamin A action. Qualitative analysis of all known RAR or RXR subtypes in both pooled and non-pooled peripheral blood mononuclear cells (PBMC) from healthy human subjects has been performed by reverse transcription and polymerase chain reaction (RT-PCR). Our data, based on qualitative RT-PCR analysis has shown that human PBMC are capable to express RAR alpha, RAR gamma, RXR alpha, and RXR beta.

Address: Institute of Experimental Endocrinology, Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovak Republic.

DOI: 10.1016/s0024-3205(02)02307-x

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MeSH Terms: Cell Nucleus, DNA Primers, Electrophoresis, Agar Gel, Humans, Leukocytes, Mononuclear, RNA, Messenger, Receptors, Retinoic Acid, Reverse Transcriptase Polymerase Chain Reaction