Dissection of nucleophilic and acid-base catalysis in glycosidases.

D L Zechel, S G Withers

Journal: Current opinion in chemical biology 2002;5(6):643-9

PMID: 11738173

Full Text on Elsevier Science View on PubMed

Abstract

A startling array of added anions have been observed to function as replacement catalytic nucleophiles in mutant glycosidases, including formate, azide, fluoride and other halides. Likewise, the mechanism of acid-base catalysis is somewhat plastic. The carboxylic acids can be substituted by a sulfenic acid or by ascorbate, and the effective acid strength enhanced by the introduction of strong hydrogen bonds. These studies provide an interesting bridge between enzymes and models thereof.

Address: Protein Engineering Network of Centres of Excellence of Canada and Department of Chemistry, University of British Columbia, Vancouver, Canada.

DOI: 10.1016/s1367-5931(01)00260-5

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MeSH Terms: Azides, Carboxylic Acids, Catalysis, Fluorides, Formates, Glycoside Hydrolases, Muramidase, Mutation, Xylan Endo-1,3-beta-Xylosidase, Xylosidases