Human neuroblastoma cell differentiation requires protein kinase C-theta.

B Sparatore, M Patrone, M Passalacqua, M Pedrazzi, S Pontremoli, E Melloni

Journal: Biochemical and biophysical research communications 2001;279(2):589-94

PMID: 11118330

Full Text on Elsevier Science View on PubMed

Abstract

Neuroblastoma LAN-5 cells exposed to retinoic acid cease to multiply and extend neurite outgrowths acquiring a neuronal phenotype. We now report that protein kinase C-theta; (PKC-theta;) isozyme is involved in this differentiation process due to the following findings: (i) PKC-theta; is expressed by LAN-5 cells as a nuclear and perinuclear protein; (ii) cell stimulation with retinoic acid promotes in a large increase in the expression level of the kinase and its intracellular redistribution; and (iii) a PKC-theta; antisense oligonucleotide reduces at the same time the expression level of the kinase and the cell response to retinoic acid. Altogether these data are consistent with a specific role played by PKC-theta; in the differentiation program of neuronal cells.

Address: Department of Experimental Medicine, Biochemistry Section, University of Genoa, Viale Benedetto XV, Genoa, 1-16132, Italy. [email protected]

DOI: 10.1006/bbrc.2000.3950

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MeSH Terms: Cell Differentiation, Cell Nucleus, Gene Expression Regulation, Enzymologic, Humans, Isoenzymes, Neuroblastoma, Oligodeoxyribonucleotides, Antisense, Polymerase Chain Reaction, Protein Kinase C, Protein Kinase C-theta, RNA, Messenger, Transcription, Genetic, Tretinoin, Tumor Cells, Cultured