A gold nanoparticle-dye/poly(3-aminobenzylamine)/two dimensional MoSe/graphene oxide electrode towards label-free electrochemical biosensor for simultaneous dual-mode detection of cancer antigen 15-3 and microRNA-21.

Chammari Pothipor, Suwussa Bamrungsap, Jaroon Jakmunee, Kontad Ounnunkad

Journal: Colloids and surfaces. B, Biointerfaces 2022;210():112260

PMID: 34894598

Full Text on Elsevier Science View on PubMed

Abstract

A dual-mode electrochemical biosensor is successfully developed for simultaneous detection of two different kinds of breast cancer biomarkers, namely cancer antigen 15-3 (CA 15-3) and microRNA-21 (miRNA-21), for the first time. The sensor composes of a poly(3-aminobenzylamine)/two-dimensional (2D) molybdenum selenide/graphene oxide nanocomposite modified two-screen-printed carbon electrode array (dual electrode), functionalized individually with 2,3-diaminophenazine-gold nanoparticles and toluidine blue-gold nanoparticles. Both kinds of the redox probe-gold nanoparticles are employed as signaling molecules and supports for immobilization of anti-CA 15-3 antibodies and capture DNA-21 probes, respectively. Due to the good conductivity and high surface-to-volume ratio of the nanocomposite, high amount of the antibodies and capture probes can be immobilized on the modified dual-electrode, giving the efficient duplex detection. Consequently, the biosensor provides good selectivity, and high sensitivity for the dual target analyte detection. The experimental results show that this label-free biosensor exhibits good linear responses to the concentrations of both target analytes with the limits of detection (LODs) of 0.14 U mL and 1.2 fM for CA 15-3 and miRNA-21, respectively. This assay strategy has a great potential to be further developed for the simultaneous detection of a variety of miRNAs and protein biomarkers for point-of-care (POC) diagnostic applications.

Address: Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; The Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.; National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.; Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand.; Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: [email protected].

DOI: 10.1016/j.colsurfb.2021.112260

Link outs

Free resources

Medical:

https://medlineplus.gov/breastcancer.html

Miscellaneous:

https://assays.cancer.gov/CPTAC-146

Research Materials:

https://antibodies.cancer.gov/detail/CPTC-MUC1-1

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Full Text Sources:

https://linkinghub.elsevier.com/retrieve/pii/S0927...

MeSH Terms: Biomarkers, Tumor, Biosensing Techniques, Breast Neoplasms, Electrochemical Techniques, Electrodes, Female, Gold, Graphite, Humans, Limit of Detection, Metal Nanoparticles, MicroRNAs, Mucin-1