Staphylococcus aureus surface protein G (sasG) allelic variants: correlation between biofilm formation and their prevalence in methicillin-resistant S. aureus (MRSA) clones.

Anna Carrera-Salinas, Aida González-Díaz, Daniel Antonio Vázquez-Sánchez, Mariana Camoez, Jordi Niubó, Jordi Càmara, Carmen Ardanuy, Sara Martí, M Ángeles Domínguez

Journal: Research in microbiology 2022;173(3):103921

PMID: 35091030

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Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) may persist for long periods due to biofilm formation. The objective of this study was to describe biofilm formation in association with the presence of S. aureus surface protein G (sasG) and its allelic variants in MRSA bacteraemia isolates from endemic (CC5, CC8, CC22) and sporadic clones in Spain (2008-2015). Crystal violet staining was used to assess biofilm formation; DNA microarray, RT-qPCR, and long-read whole genome sequencing were applied to determine the presence, expression and structure of sasG, respectively. The endemic CC5 and CC8 clones produced more biofilm than the sporadic clones; these endemic clones carried sasG allelic variant 1. Otherwise, sporadic clones, with less biofilm formation, showed either an absence of sasG (65%) or the presence of allelic variant 2 (35%). Variants 1 and 2 differed in the expression of sasG (1.56 ± 1.20 and 0.37 ± 0.32, respectively). The analysis of a large cohort of closed S. aureus genomes available on the NCBI database confirmed the distribution of the two allelic variants with low amino acid identity (68.1%) among endemic and sporadic clones. SasG variant 1 present in the major CC5 and CC8 clones was correlated with increased biofilm formation and may represent an important virulence determinant.

Address: Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Infectious Diseases (CIBERINFEC), ISCIII, Madrid, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Infectious Diseases (CIBERINFEC), ISCIII, Madrid, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain; Department of Pathology and Experimental Therapeutics, College of Medicine, Universitat de Barcelona, Barcelona, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Respiratory Diseases (CIBERES), ISCIII, Madrid, Spain. Electronic address: [email protected].; Department of Microbiology, Hospital Universitari de Bellvitge, Institut D'Investigació Biomèdica de Bellvitge (IDIBELL), Barcelona, Spain; Research Network for Infectious Diseases (CIBERINFEC), ISCIII, Madrid, Spain; Department of Pathology and Experimental Therapeutics, College of Medicine, Universitat de Barcelona, Barcelona, Spain. Electronic address: [email protected].; Hospital Universitari Arnau de Vilanova, Lleida, Spain.; Hospital Universitari Clínic, Barcelona, Spain.; Hospital Universitario 12 de Octubre, Madrid, Spain.; Hospital General Universitario Gregorio Marañón, Madrid, Spain.; Hospital Universitari Joan XXIII, Tarragona, Spain.; Hospital Universitari Mútua de Terrassa, Terrassa, Spain.; Hospital Universitari Parc Taulí, Sabadell, Spain.; Hospital Universitario Ramón y Cajal, Madrid, Spain.; Hospital Universitario Reina Sofía, Córdoba, Spain.; Hospital San Pedro de La Rioja, Logroño, Spain.; Hospital de La Santa Creu I Sant Pau, Barcelona, Spain.; Hospital Universitari Son Espases, Mallorca, Spain.; Hospital Universitario Virgen Del Rocío, Sevilla, Spain.; Hospital Universitari Vall D'Hebron, Barcelona, Spain.

DOI: 10.1016/j.resmic.2022.103921

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MeSH Terms: Anti-Bacterial Agents, Biofilms, Clone Cells, Humans, Membrane Proteins, Methicillin Resistance, Methicillin-Resistant Staphylococcus aureus, Microbial Sensitivity Tests, Prevalence, Staphylococcal Infections, Staphylococcus aureus