A Mini-review on Potentials Proteins/Peptides Applied for Serodiagnosis of Human Monkeypox Infection and Future Trends.

Fellipe Alexandre Alves Moraes, Caíque Lopes Duarte, Gabriel Oliveira Fernandes, Lucas Antônio Lisboa Ribeiro, Luciana Martins Rodrigues, Junivania Aparecida Dos Santos Lacerda, Líria Souza Silva, Jonatas Oliveira da Silva, Michelli Dos Santos, Alessandra da Silva Dantas, Miguel Angel Chávez-Fumagalli, Mariana Campos-da-Paz, Rodolfo Cordeiro Giunchetti, Eduardo Antônio Ferraz Coelho, Juliana Martins Machado, Alexsandro Sobreira Galdino

Journal: Protein and peptide letters 2023;30(5):374-383

PMID: 36998139

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Abstract

Monkeypox is a zoonosis that re-emerged in 2022, generating cases in non-endemic countries for the disease and creating a public health issue. The rapid increase in the number of cases kindles a need for quick, inexpensive diagnostic tests for the epidemiological control of the disease. The high cost of molecular tests can make this control more difficult to access in poorer regions, with immunological tests being a more viable option. In this mini-review, a search was conducted in the main databases for peptide and protein options that could be used in the development of serological diagnostic tests. Nine viable registres were found, and seven were selected (two patents and five studies). The main studies used the B21R peptide sequence as it is a high immunogenic epitope. In addition, studies on the improvement of these sequences were also found to avoid cross-reactions against other viruses of the same family, proposing a rational approach using multiepitope recombinant proteins. These approaches demonstrated high sensitivity and specificity values and are seen as viable options for developing new tests. New effective serological testing options, when combined with awareness, disease surveillance, early diagnosis, and rapid communication, form a set of key strategies used by health systems to control the spread of the monkeypox virus.

Address: Department of Pharmacy, Disciplina Biotecnologia & Inovações, Programa de Pós-graduação em Biotecnologia e Programa Multicentrico de Pós-graduação em Bioquímica e Biologia Molecular, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.; Department of Biochemistry, Laboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.; School of Dental Sciences, Newcasttle University, Flalington Place, New Casttle upon Tyne, New Casttle, UK.; Computational Biology and Chemistry Research Group, Vicerrectorado de Investigación, Universidad Católica de Santa María, Arequipa, Peru.; Department of Biochemistry, Laboratório de Bioativos e Nanobiotecnologia, Universidade Federal de São João Del-Rei, Divinópolis, MG, 35501-296, Brazil.; Department of Morphology, Laboratory of Cell-Cell Interactions, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil.; Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, 30130-100, Minas Gerais, Brazil.

DOI: 10.2174/0929866530666230328094425

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MeSH Terms: Humans, Mpox, Monkeypox, Peptides, Amino Acid Sequence, Recombinant Proteins, Serologic Tests