In Vitro and in Vivo Assays for Testing Retinoids Effect on Intestinal Progenitors' Lineage Commitments.

Krishna R Gajera, Kathryn L Fair, Gordon W Moran, Nicholas R F Hannan, Joerg Huelsken, Paloma Ordóñez-Morán

Journal: Methods in molecular biology (Clifton, N.J.) 2023;2650():53-61

PMID: 37310623

Abstract

The intestine consists of epithelial cells surrounded by a complex environment as mesenchymal cells and the gut microbiota. With its impressive stem cell regeneration capability, the intestine is able to constantly replenish cells lost through apoptosis or abrasion by food passing through. Over the past decade, researchers have identified signaling pathways involved in stem cell homeostasis such as retinoids pathway. Retinoids are also involved in cell differentiation of healthy and cancer cells. In this study, we describe several approaches in vitro and in vivo to further investigate the effect of retinoids on stem cells, progenitors, and differentiated intestinal cells.

Address: Swiss Institute for Experimental Cancer Research (ISREC), École Polytechnique Fédérale de Lausanne-(EPFL-SV), Lausanne, Switzerland.; Translational Medical Sciences Unit, School of Medicine, Centre for Cancer Sciences, Biodiscovery Institute-3, University Park, University of Nottingham, Nottingham, UK.; Nottingham Digestive Diseases Centre, University of Nottingham, Nottingham, UK.; NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust, Nottingham, UK.; Translational Medical Sciences Unit, School of Medicine, Centre for Cancer Sciences, Biodiscovery Institute-3, University Park, University of Nottingham, Nottingham, UK. [email protected].

DOI: 10.1007/978-1-0716-3076-1_5

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https://doi.org/10.1007/978-1-0716-3076-1_5

MeSH Terms: Apoptosis, Biological Assay, Cell Differentiation, Intestines, Retinoids